Virtual Saturation Mutagenesis to Map Critical Functional Regions

Local mutation scans and structural review identify region-level hypotheses rather than claiming successful single substitutions. The graphical abstract brings together the principal sequence, structure and property results across candidates. The combined evidence establishes actionable priorities for sequences, sites or peptide candidates. The result-focused presentation supports efficient review of the main evidence and research priorities. It also supports focused comparison across the disclosed computational results.